we speak peptides
WHAT WE DO FOR YOU
Research projects
ProteoFormiX developed proprietary top-down proteomics and mass spectrometry imaging-based technology that is elegantly suited for biomarker discovery. We prefer to engage in research projects with biomedical/pharmaceutical partners who are experts in diseases with a high unmet medical need today, and are committed to their success.
Typical projects carried out so far include thorough comparative analyses of proteoforms
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present in healthy versus diseased samples,
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in clinical as well as pre-clinical samples,
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collected from subjects untreated versus treated, with placebo, with drugs, or with drug candidate molecules.
Unique top-down approach
No disease or therapeutic area exists in which ProteoFormiX’ technology cannot be applied. Unique in ProteoFormiX' proprietary analytical approach is that it allows for specific translational biomarker discovery directly from FFPE (formaldehyde-fixed, paraffin-embedded) clinical tissues, such as those biobanked in well-documented pathology archives all over the planet. This is of particular interest for diseases which today can only be unambiguously diagnosed in biopsy or post mortem material.
The primary areas of focus in which ProteoFormiX is currently active on a project basis, with its unique top-down mass spectrometry identification and analysis approach, are:
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neuronal diseases (various types of neurodegeneration, multiple sclerosis, …)
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oncology (colon, breast, urogenital cancers, pituitary adeno-carcinoma, …)
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infectious (in particular pulmonary) diseases
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eye specific disorders (dry eye disease, glaucoma, …)
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as well as metabolic diseases (diabetes, …).
Mass spectrometry imaging analyses
ThermoFisher LTQ Orbitrap Velos fitted with MassTech UHR AP/MALDI source
ThermoFisher nano ESI LTQ Orbitrap Velos interfaced on-line with Agilent 1200 HPLC
Denator Stabilizor T1
ThermoFisher LTQ Orbitrap Velos fitted with MassTech UHR AP/MALDI source
ProteoFormiX possesses its own high-resolution mass spectrometry (MS) instrumentation and know-how to offer specialized mass spectrometry imaging analyses with focus on, but not restricted to FFPE samples. Although focusing on top-down MS analyses to characterize biomolecules (endogenous peptides, proteins and metabolites in particular), we also do classical bottom-up or shot gun proteomics services. For the latter our close collaboration with the Laboratory for Applied Mass Spectrometry at the Department of Cellular and Molecular Medicine of the University of Leuven is invaluable.